Journal: Journal for Immunotherapy of Cancer

Article Title: 31st Annual Meeting and Associated Programs of the Society for Immunotherapy of Cancer (SITC 2016): part two

doi: 10.1186/s40425-016-0173-6

Figure Lengend Snippet: a Distribution (%) of 186 patients with gastric cancer according to the expression of PD-L1 on tumor cells (TC) and tumor-infiltrating immune cells (IC). b PD-L1 expression by tumor cells (TC) and tumor-infiltrating immune cells (IC) is reduced with fewer infiltrating CD8+ T cells. c Distribution (%) of tumor microenvironment immune type based on the presence of tumor-infiltrating CD8+ T cells and PD-L1 status. Tumor-infiltrating CD8+ T cell IHC score=1-3 were considered positive. PD-L1 in tumor samples by TC1/2/3 or IC1/2/3 was considered positive

Article Snippet: To identify potentially synergistic immunotherapies, we evaluated AXAL ± antibodies for T cell co-inhibitory or co-stimulatory receptors (checkpoint inhibitors: CTLA-4, PD-1, TIM-3, LAG-3; co-stimulators: CD137, OX40, GITR, and CD40) in a mouse HPV-positive tumor model. Methods C57BL/6 female mice and TC1 cells (C57BL/6 mouse lung epithelial cells co-transfected with HPV16 E6 and E7 and activated Ras ) were obtained from Jackson Laboratories and ATCC, respectively.

Techniques: Expressing

Journal: Genetics

Article Title: Identification of trans Protein QTL for Secreted Airway Mucins in Mice and a Causal Role for Bpifb1

doi: 10.1534/genetics.117.300211

Figure Lengend Snippet: MUC5AC and MUC5B expression in CC founder strains. Immunoblots for (A) MUC5AC and (B) MUC5B in BALF of CC founder strains sensitized and challenged with Der p 1 (A/J challenged with PBS or Der p 1), with n = 3–4 mice/strain. + denotes BALF sample from a C57BL/6J mouse treated with allergen in a separate experiment and used as positive control for antibody reactivity. Quantification of protein signal (integrated intensity a.u.) for (C) MUC5AC and (D) MUC5B show significant effect of strain as calculated by ANOVA for MUC5AC (P strain = 4.0 × 10−7) and MUC5B (P strain = 1.1 × 10−5). Each circle represents one mouse and the diamonds indicate strain means.

Article Snippet: We found that the expression of both proteins increased significantly as a function of allergen ( ) in a highly correlated fashion ( r = 0.98, P < 0.001, ). table ft1 table-wrap mode="anchored" t5 caption a7 Differential expression: fold change vs. control GEO ID Array platform Mouse strain Allergen Tissue n treat / n control Muc5b P -value Bpifb1 P -value {"type":"entrez-geo","attrs":{"text":"GSE1301","term_id":"1301"}} GSE1301 a Affymetrix mouse expression 430A array Not reported HDM Whole lung 3/3 2.68 6.73E−05 2.67 8.56E−03 {"type":"entrez-geo","attrs":{"text":"GSE13032","term_id":"13032"}} GSE13032 b Affymetrix mouse genome 430 2.0 array A/J and C57BL/6J OVA Whole lung 3/3 A/J, 3/3 C57BL/6J 2.66 1.62E−06 1.86 1.28E−06 {"type":"entrez-geo","attrs":{"text":"GSE13382","term_id":"13382"}} GSE13382 Agilent-014868 whole mouse genome microarray 4x44K Not reported OVA Whole lung 3/3 1.48 6.87E−02 1.61 3.61E−02 {"type":"entrez-geo","attrs":{"text":"GSE1438","term_id":"1438"}} GSE1438 Custom arrays BALB/c OVA Whole lung 5/5 1.55 2.50E−01 1.60 1.10E−01 {"type":"entrez-geo","attrs":{"text":"GSE19223","term_id":"19223"}} GSE19223 b Affymetrix mouse exon 1.0 ST array C57BL/6J and BALB/cJ HDM Whole lung 11/12 C57BL/6J, 11/14 BALB/cJ 2.21 7.45E−03 3.77 2.11E−03 {"type":"entrez-geo","attrs":{"text":"GSE27066","term_id":"27066"}} GSE27066 c Affymetrix mouse genome 430 2.0 array C57BL/6 OVA Whole lung 4/4 −1.14 4.27E−03 −1.43 5.21E−01 {"type":"entrez-geo","attrs":{"text":"GSE34764","term_id":"34764"}} GSE34764 Affymetrix mouse gene 1.0 ST array C57BL/6 Alternaria Airway epithelia 3/3 1.38 3.05E−02 1.21 1.02E−02 {"type":"entrez-geo","attrs":{"text":"GSE6858","term_id":"6858"}} GSE6858 Affymetrix mouse genome 430 2.0 array BALB/cJ OVA Whole lung 4/4 3.89 5.74E−04 2.67 9.64E−03 Meta-analysis P -value 1.48E−13 1.81E−10 Open in a separate window a Some studies included additional mice with engineered mutations which were not included in this meta-analysis. b Analysis adjusted for strain. c No upregulation of Muc5ac detected in this study. caption a8 Meta-analysis of Muc5b and Bpifb1 gene expression in previous studies fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window caption a7 Correlated expression of MUC5B and BPIFB1 in airway epithelium.

Techniques: Expressing, Western Blot, Positive Control

Journal: Genetics

Article Title: Identification of trans Protein QTL for Secreted Airway Mucins in Mice and a Causal Role for Bpifb1

doi: 10.1534/genetics.117.300211

Figure Lengend Snippet: Meta-analysis of Muc5b and Bpifb1 gene expression in previous studies

Article Snippet: We found that the expression of both proteins increased significantly as a function of allergen ( ) in a highly correlated fashion ( r = 0.98, P < 0.001, ). table ft1 table-wrap mode="anchored" t5 caption a7 Differential expression: fold change vs. control GEO ID Array platform Mouse strain Allergen Tissue n treat / n control Muc5b P -value Bpifb1 P -value {"type":"entrez-geo","attrs":{"text":"GSE1301","term_id":"1301"}} GSE1301 a Affymetrix mouse expression 430A array Not reported HDM Whole lung 3/3 2.68 6.73E−05 2.67 8.56E−03 {"type":"entrez-geo","attrs":{"text":"GSE13032","term_id":"13032"}} GSE13032 b Affymetrix mouse genome 430 2.0 array A/J and C57BL/6J OVA Whole lung 3/3 A/J, 3/3 C57BL/6J 2.66 1.62E−06 1.86 1.28E−06 {"type":"entrez-geo","attrs":{"text":"GSE13382","term_id":"13382"}} GSE13382 Agilent-014868 whole mouse genome microarray 4x44K Not reported OVA Whole lung 3/3 1.48 6.87E−02 1.61 3.61E−02 {"type":"entrez-geo","attrs":{"text":"GSE1438","term_id":"1438"}} GSE1438 Custom arrays BALB/c OVA Whole lung 5/5 1.55 2.50E−01 1.60 1.10E−01 {"type":"entrez-geo","attrs":{"text":"GSE19223","term_id":"19223"}} GSE19223 b Affymetrix mouse exon 1.0 ST array C57BL/6J and BALB/cJ HDM Whole lung 11/12 C57BL/6J, 11/14 BALB/cJ 2.21 7.45E−03 3.77 2.11E−03 {"type":"entrez-geo","attrs":{"text":"GSE27066","term_id":"27066"}} GSE27066 c Affymetrix mouse genome 430 2.0 array C57BL/6 OVA Whole lung 4/4 −1.14 4.27E−03 −1.43 5.21E−01 {"type":"entrez-geo","attrs":{"text":"GSE34764","term_id":"34764"}} GSE34764 Affymetrix mouse gene 1.0 ST array C57BL/6 Alternaria Airway epithelia 3/3 1.38 3.05E−02 1.21 1.02E−02 {"type":"entrez-geo","attrs":{"text":"GSE6858","term_id":"6858"}} GSE6858 Affymetrix mouse genome 430 2.0 array BALB/cJ OVA Whole lung 4/4 3.89 5.74E−04 2.67 9.64E−03 Meta-analysis P -value 1.48E−13 1.81E−10 Open in a separate window a Some studies included additional mice with engineered mutations which were not included in this meta-analysis. b Analysis adjusted for strain. c No upregulation of Muc5ac detected in this study. caption a8 Meta-analysis of Muc5b and Bpifb1 gene expression in previous studies fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window caption a7 Correlated expression of MUC5B and BPIFB1 in airway epithelium.

Techniques: Expressing, Microarray

Journal: Genetics

Article Title: Identification of trans Protein QTL for Secreted Airway Mucins in Mice and a Causal Role for Bpifb1

doi: 10.1534/genetics.117.300211

Figure Lengend Snippet: Correlated expression of MUC5B and BPIFB1 in airway epithelium. Der p 1 challenge elicits a correlated increase in MUC5B and BPIFB1 in allergen-sensitized C57BL/6J mice. (A) Large airway staining shows increase in AB-PAS, MUC5B, and BPIFB1 in airway epithelium. Images representative of group based on average quantified signal. Bar, 100 μM. (B) Quantitative morphometry of total airway signal (Vs, mean ± SEM) for PBS control (light gray) or Der p 1 treated (dark gray) mice. n = 5–6 mice/treatment group. Fold change for treatment: 6.2 (AB-PAS), 5.4 (MUC5B), 4.5 (BPIFB1). Significance of two-sided t-tests of treatment indicated as *** P < 0.001. (C) Correlation of BPIFB1 and MUC5B total airway signal. Pearson’s correlation r = 0.98 (P < 0.001). Mice challenged with PBS (light gray) or Der p 1 (dark gray).

Article Snippet: We found that the expression of both proteins increased significantly as a function of allergen ( ) in a highly correlated fashion ( r = 0.98, P < 0.001, ). table ft1 table-wrap mode="anchored" t5 caption a7 Differential expression: fold change vs. control GEO ID Array platform Mouse strain Allergen Tissue n treat / n control Muc5b P -value Bpifb1 P -value {"type":"entrez-geo","attrs":{"text":"GSE1301","term_id":"1301"}} GSE1301 a Affymetrix mouse expression 430A array Not reported HDM Whole lung 3/3 2.68 6.73E−05 2.67 8.56E−03 {"type":"entrez-geo","attrs":{"text":"GSE13032","term_id":"13032"}} GSE13032 b Affymetrix mouse genome 430 2.0 array A/J and C57BL/6J OVA Whole lung 3/3 A/J, 3/3 C57BL/6J 2.66 1.62E−06 1.86 1.28E−06 {"type":"entrez-geo","attrs":{"text":"GSE13382","term_id":"13382"}} GSE13382 Agilent-014868 whole mouse genome microarray 4x44K Not reported OVA Whole lung 3/3 1.48 6.87E−02 1.61 3.61E−02 {"type":"entrez-geo","attrs":{"text":"GSE1438","term_id":"1438"}} GSE1438 Custom arrays BALB/c OVA Whole lung 5/5 1.55 2.50E−01 1.60 1.10E−01 {"type":"entrez-geo","attrs":{"text":"GSE19223","term_id":"19223"}} GSE19223 b Affymetrix mouse exon 1.0 ST array C57BL/6J and BALB/cJ HDM Whole lung 11/12 C57BL/6J, 11/14 BALB/cJ 2.21 7.45E−03 3.77 2.11E−03 {"type":"entrez-geo","attrs":{"text":"GSE27066","term_id":"27066"}} GSE27066 c Affymetrix mouse genome 430 2.0 array C57BL/6 OVA Whole lung 4/4 −1.14 4.27E−03 −1.43 5.21E−01 {"type":"entrez-geo","attrs":{"text":"GSE34764","term_id":"34764"}} GSE34764 Affymetrix mouse gene 1.0 ST array C57BL/6 Alternaria Airway epithelia 3/3 1.38 3.05E−02 1.21 1.02E−02 {"type":"entrez-geo","attrs":{"text":"GSE6858","term_id":"6858"}} GSE6858 Affymetrix mouse genome 430 2.0 array BALB/cJ OVA Whole lung 4/4 3.89 5.74E−04 2.67 9.64E−03 Meta-analysis P -value 1.48E−13 1.81E−10 Open in a separate window a Some studies included additional mice with engineered mutations which were not included in this meta-analysis. b Analysis adjusted for strain. c No upregulation of Muc5ac detected in this study. caption a8 Meta-analysis of Muc5b and Bpifb1 gene expression in previous studies fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window caption a7 Correlated expression of MUC5B and BPIFB1 in airway epithelium.

Techniques: Expressing, Staining